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KMID : 0615720120290060011
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Jouranl of Korean Acupuncture and Moxibustion society
2012 Volume.29 No. 6 p.11 ~ p.21
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Effects of Astragalus Membranaceus, Innamomum Cassia, Phellodendron Amurensis(BHH10) on MC3T3-E1 Cells Proliferation, Differntiation and Bone Mineralized Formation
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Lee Mi-Lim
Hur Jeong-Eun
Nam Dong-Woo
Kang Jung-Won
Kim Sung-hoon
Choi Do-Young
Lee Jae-Dong
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Abstract
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Objectives : BHH10 is traditional medicine herb used for enhancing body resistance against various diseases. The aim of this study was to identify BHH10 extract induces osteogenic activity in human osteoblast-like MC3T3-E1 cells.
Methods : MC3T3-E1, pre-osteoblast cell line, were treated with BHH10 of various concentrations($0.1{\mu}g/mL$ 수식 이미지, $1{\mu}g/mL$ 수식 이미지, $10{\mu}g/mL$ 수식 이미지). And then, the effect of BHH10 on osteoblast differentiation was examined by alkaline phosphatase(ALP) activity, von Kossa staining and RT-PCR for osteoblast differentiation markers such as osteocalcin(OCN), osteopontin(OPN).
Results : BHH10 had dose-dependent effect on the viability of osteoblastic cells, and dose-dependently increased alkaline phosphatase(ALP) activity. BHH10 markedly increased mRNA expression for OCN, OPN in MC3T3-E1 cells. Also, BHH10 significantly induced mineralization in the culture of MC3T3-E1 cells.
Conclusions : In conclusion, these results propose that BHH10 can play an important role in osteoblastic bone formation, osteogenesis, and may possibly lead to the development of bone-forming drugs.
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KEYWORD
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BHH10 , ALP activity , OCN , OPN , mineralization
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